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1·Methods 30 ampuls in the experiment group were opened by sterilized tweezers. The other 30 ampuls in control group were opened by hand. Liguid in each ampul was cultiuated immediately.

方法将消毒瓶镊折断安瓿方法为实验组，将手折断安瓿方法为对照组，各折断安瓿30支后进行药液培养。