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1·The experimental results show that when adopting MS basal medium, adding 2,4-D benefit to induce callus;

实验结果表明：以MS为基本培养基，在愈伤诱导培养基中加入2，4-D利于愈伤组织的诱导；

2·In vitro propagation of garlic was studied by using regular varieties as material and B5 as the basal medium.

以常规大蒜栽培品种为试材，B5为基本培养基，研究了利用脱毒大蒜萌生幼苗的离体繁殖技术。

3·The results showed that the effects of basal medium, IBA and dark or light culture were significant at 1% level.

结果表明基本培养基、IBA和光暗培养是影响试管苗产生不定根的主要因素。

4·Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.

胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。

5·Their stem explants were grown in MS or modified MS basal medium. The effects of plant hormones on organogenesis and transplanting of test-tube plantlets were studied.

茎段培养在MS或改良MS基本培养基中，研究植物激素对器官形成的影响及试管苗移栽技术。

6·With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA, IBA , 2,4-D.

以大叶黄杨的幼茎段为外植体，在添加BA和NAA、IBA、2，4-D不同激素组合的MS培养基上培养，对其愈伤组织进行诱导试验。

7·Method: MS medium was used as basal medium and ba and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting.

方法：以MS培养基为基本培养基，通过研究植物生长物质BA和NAA对丛生芽的分化、丛生芽的增殖和生根的影响找出相应的最佳生长物质组合。

8·The percentage of yeast extract of the nitrogen source during the growth phase and in the basal medium during the phase of induction and the time and condition that the induction starts are effective.

结果表明，基础料和补充氮源中酵母膏的比例、诱导时机和乳糖流加方式等对表达量的提高有明显的影响。

9·CONCLUSION: the relatively pure and undifferentiated limbal stem cells can be obtained by combining non calcium medium and low calcium medium after the basal limbal cell tissues are cultured.

结论：取用角膜缘基底组织进行培养，将无钙培养液与低钙培养基结合，可获得较纯的、未分化的角膜缘干细胞。

10·Methods Limbal button with basal cells was cultured in low calcium medium.

方法采用低钙培养法对人角膜缘基底层细胞组织块进行培养。