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1·Suspension single cells were obtained after successive subculturing of 3 or 4 generations.

经3～ 4次继代培养即可得到悬浮的单细胞。

2·Sectoring became visible after subculturing for 5-6 generations, more frequently with 13s8.

菌株角变现象在继代培养5 - 6代后表现明显，而13s8角变子出现的频更高。

3·The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.

而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。