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1·The fed-batch fermentation method was studied by flask culture based on the optimal medium.

然后在该培养基配方的基础上进行摇瓶分批补料发酵试验，确定最佳补料方案。

2·Method: Based on enrichment culture, blood plate separation, the flask culture and exclusive circle of methods such as oil.

方法：富集培养、血平板分离、摇瓶培养和排油圈测定。

3·We prepared 6.58mg soluble MGL from 1L shake-flask culture with higher activity, with a 41.1% overall yield in the purification process.

活性测定显示可溶性MGL具有较高活性，每升发酵菌液可以制备6.58毫克可溶性MGL，纯化回收率达到41.1%。

4·Objective:In this article, the shaking flask culture conditions of strain SD59 which had a high productive capacity of lipopeptide biosurfactant have been studied.

目的：对选育出的脂肽生物表面活性剂高产菌株SD59的摇瓶发酵条件进行了研究。

5·Objective To investigate the influences of oxygen vector n-hexadecane on Xanthophyllomyces dendrorhous growth and astaxanthin production in the shake-flask culture.

目的 考察氧载体正十六烷对红法夫酵母发酵产虾青素的影响。

6·Through flask culture, research Lentinula edods submerged culture optimum fermentation time, the optimum carbon, nitrogen and optimum best quality carbon and nitrogen ratio.

通过摇瓶培养，研究香菇液体深层培养最适发酵培养时间、最适碳源、最适氮源及最佳碳源与氮源质量配比。

7·The optimum flask culture conditions of rhamnolipid biosurfactant production from refined vegetable oil wastewater were investigated by using a Pseudomonas aeruginosa strain O-2-2.

通过摇瓶培养试验，研究了铜绿假单胞菌O-2-2以植物油精炼废水为原料生产鼠李糖脂生物表面活性剂的适宜条件。

8·Shaking flask culture is being widely used for the evaluation of a new antibiotic producing strain in screening programme or to ascertain the culture condition of a certain microorganism.

摇瓶培养被广泛用于新抗生素菌种的筛选和选育以及培养条件的确定。

9·Shake flask culture was used and the yield of polysaccharide was taken as main index to ensure the optimum culture conditions of submerged fermentation for Ganoderma lucidum by corn mash medium.

以灵芝多糖为指标，采用摇瓶液体发酵的方法，研究了以玉米水解糖为主要原料，液体培养灵芝的发酵条件。

10·To demonstrate their approach, they added the genes to a bacterium and found that a flask of the bacterial culture produced enough light to read a book by.

为了展示他们的方法，他们给一种细菌添加了这种基因，并发现培养细菌的烧瓶中放出的光足可以在旁边看书。