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1·Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.

采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。

2·Methods Gradient centrifugation and adherent culture were used to get a great amount of NSC and then differentiation antigens were identified.

方法用梯度离心和贴壁培养法分离msc，进行表面分化抗原的鉴定。

3·Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.

无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。

4·Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.

结论改良全骨髓贴壁法可有效分离培养大鼠的骨髓间充质干细胞，是一种比较理想的分离培养方法。