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1·Method Immunohistochemical staining technique.

方法免疫组织化学法。

2·Morphological changes of brain were observed by HE staining technique;

HE染色观察脑组织形态学变化；

3·Methods Double labeling immunocytochemical staining technique was used.

方法采用免疫细胞化学双重标记技术。

4·Methods Flow cytometry and immunofluorescence staining technique were used.

方法：采用细胞免疫荧光染色技术和流式细胞术。

5·Methods: The double labeling immunocytochemical staining technique was used.

方法：免疫细胞化学双重标记技术。

6·Method Immunocytochemical staining technique by using specific antibody against P2X4 receptor.

方法P 2x4受体特异性抗体的免疫细胞化学染色。

7·Objective to evaluate the method of study on lymphatic capillaries in the liver using the immunohistochemical staining technique.

目的评价免疫组织化学染色法在肝脏淋巴管研究中的作用。

8·Objective To evaluate the method of study on lymphatic capillaries in the liver using the immunohistochemical staining technique.

结论：免疫组织化学染色法在肝脏毛细淋巴管的研究中是一种较为准确、观的方法。

9·Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.

方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。

10·METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.

方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。