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1·Step 3: Elute glutathione resin-bound GTPase with SDS buffer.

第三步：使用SDS缓冲液稀释谷胱甘肽树脂。

2·ADP ribosylation factors (ARFs) are critical in the vesicular trafficking pathway. ARF activity is controlled by GTPase-activating proteins (GAPs).

ADP核糖基化因子(ARF)在膜泡运输中具有重要作用，其活性受gtp酶活化蛋白(gap)的调控。

3·Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.

目的建立甲基化特异性PCR（MSP）技术检测黏着斑激酶相关性鸟苷三磷酸酶调节因子（GRAF）基因启动子甲基化。