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1·PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.

从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。

2·After subculture, confluence was occurred in 1-2 weeks.

传代培养细胞可在1 ~ 2周汇合成片。

3·Anime subculture has a distinctive effect on juvenile delinquency.

青少年亚文化对青少年犯罪的影响极为显着。

4·Websites devoted to the phones are appearing and have become a genuine subculture.

为山寨机设立的网站不断出现，成为一种真正的亚文化现象。

5·However, to brand culture, subculture is an important carrier of communication.

而对于品牌文化来说，亚文化是一个重要的传播载体。

6·Once simply a bizarre subculture in Japan, cosplay is becoming a global phenomenon.

动漫真人秀曾经还只是一种标新立异的日本亚文化，而今正在成为一种全球现象。

7·The unhealthy subculture of college students has its basic form and characteristics.

大学生不良亚文化有其基本的表现形式和特点。

8·Freedom isn't about a subculture; it's about the culture. It's not a microcosm; it's a cosm.

《自由》写的不是亚文化而是关于文化；不是微小世界而是大世界。

9·Somatic clones were established after 15 generations of subculture for more than two years.

经过两年多(15个世代)的继代培养，建立了党参体细胞无性系。

10·As a kind of subculture, Enterprise culture is also influenced by Chinese Traditional culture.

企业文化作为一种亚文化，受到传统文化的深远影响。