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1·Master basic subject test skills, for example titration, weighing materials, electrophoresis, Gram staining and sub-culture etc.

良好的专业实验技能，例如滴定操作、称量、电泳、革兰氏染色、划线培养等。

2·RESULTS The special spore of P. marneffei could be found by sodium hydroxide smear of the liquid of lymphonodus puncture and Gram staining.

结果 氢氧化钠涂片和革兰染色能检出马尔尼菲青霉菌所特有的孢子。

3·The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .

运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。