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1·At last, we got one positive hybridoma cells named 4d11.

筛选共得到一株阳性杂交瘤细胞，命名为4d11。

2·This is a reliable method to select the hybridoma cells for McAb of IBDV.

是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。

3·Then the hybridoma cell lines secreting high titer of McAbs were screened.

目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。

4·The chromosome of the positive hybridoma and the character of the mAb were analysed.

对所得阳性杂交瘤细胞作染色体分析并对单克隆抗体进行特性分析。

5·Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.

方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。

6·Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.

方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。

7·Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

通过试管法和玻片法检测抗a抗体的效价和亲和力。

8·We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.

用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。

9·The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。

10·Absorption of human-human hybridoma cells can abolish the human hybridoma growth factor (HGF) activity but not the BCGF activity.

但人-人B细胞杂交瘤可吸收掉上述BCGF活性上清中的促杂交瘤细胞生长因子(HGF)活性，而对BCGF活性无明显影响。

杂交瘤

1·The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。

2·Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.

研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。

3·This is a reliable method to select the hybridoma cells for McAb of IBDV.

是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。

4·Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.

方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。

5·Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.

目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。