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1·Mammalian primordial germ cells form and migrate to the gonad during embryonic development.

在胚胎发育时期，原始生殖细胞形成并迁移至生殖腺，形成种系囊。

2·Objective:To isolate and clone bovine embryonic stem cells from bovine fetus Primordial germ cells(PGCs).

目的从牛原始生殖细胞中分离克隆牛胚胎干细胞。

3·In vitro culture of primordial germ cells, establishment of EG cells line and their application prospect are reviewed.

本文就原始生殖细胞体外培养，建立EG细胞系及其应用前景作一综述。

4·When cultured on feeder cells in the presence of many kinds of cytokines, primordial germ cells can proliferate continually and maintain undifferentiated state.

在饲养细胞层和多种生长因子的共同作用下，可保持原生殖细胞在体外不断增殖而不分化，最终建立EG细胞系。

5·Primordial germ cells came from pole cells and moved in two masses to the laterodorsal abdomen of embryo, and then were enveloped by mesoderm to form germinal ridge.

原始生殖细胞来自极细胞，分两团移至胚胎腹部侧背面，被中胚层细胞包裹，形成生殖脊。

6·Here, we show that removing the translational repressor Nanos from either GSCs or their precursors, the primordial germ cells (PGCs), causes both cell types to differentiate into germline cysts.

作者表明从gsc或者其前体原始生殖系细胞(PGCs)去除翻译抑制物nan os将使两种细胞分化成生殖系包囊。

7·The germ line cysts break apart into individual cells and become packaged into primordial follicles.

前泡膜细胞被募集到原始卵泡周围，原始卵泡发育并向初级卵泡转化。