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1·The coating antigen in a 96 pore plate in the kit is the prokaryotic expression recombinant P30 protein, and has good antigenicity.

试剂盒中96孔板中的包被抗原为原核表达的重组P 30蛋白，其具有良好的抗原性。

2·The indirect ELISA is widely used for detection of antibodies and coating antigen is very important to the accuracy of ELISA experiment.

本试验分别使用超声波裂解抗原、脂多糖蛋白抗原和全菌抗原作为包被抗原，建立相应的检测方法并进行重复性试验。

3·An ic-ELISA for detecting fumonisin B2(FB2) of maizena was developed by using the coating antigen of OVA-FB2 and competitive antigen of FB2.

用卵清白蛋白与伏马菌素B2的偶联物（OVA-FB2）做包被抗原，标准伏马菌素B2（FB2）做竞争抗原，初步建立了检测玉米粉中伏马菌素B2的间接竞争ELISA方法。

4·OA-OVA, as the coating antigen, were prepared by activated ester method, and at last we established the indirect competitive ELISA for detecting OA.

依照活性酯法将OA与OVA偶联作为包被抗原，建立了间接竞争ELISA检测OA的方法。

5·An indirect ELISA was developed and optimized with the purified recombinant E2 protein as coating antigen. The optimal conditions of ELISA were determined as follows.

将表达的重组E2蛋白纯化后作为ELISA包被抗原，建立了一种基于重组E2蛋白的间接ELISA方法，并对此方法的反应条件进行了优化。

6·An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.

用表达的口蹄疫3a蛋白作为包被抗原，建立了间接elisa试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。

7·Objective:An indirect competitive time-resolved fluoroimmunoassay(TRFIA) was established using anti-DON polyclonal antibody and coating antigen DON-BSA to detect deoxynivalenol in cereal.

目的：利用抗脱氧雪腐镰刀烯醇多克隆抗体及其人工抗原，采用时间分辨免疫荧光技术建立间接竞争脱氧雪腐镰刀烯醇时间分辨免疫检测方法（DON-TRFIA）。

8·The kit adopts prokaryotic expression recombinant P30 protein as coating antigen, and detects the African swine fever virus antibody in pig serum according to a competitive ELISA principle.

试剂盒采用原核表达的重组P 30蛋白作为包被抗原，依据竞争ELISA原理检测猪血清中非洲猪瘟病毒的抗体。