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1·Objective To construct recombinant cosmid vector pAxCAwt MC148 from the MC148 gene of molluscum contangiosum virus(MCV).

目的构建传染性软疣病毒（MCV ）MC14 8基因腺病毒重组粘粒。

2·METHODS: Angiopoietin-1-PAxCAwt cosmid DNA and adenoviral DNA-TPC were cotransfected to 293 cells by the use of calcium phosphate precipitation method.

方法：人血管形成素- 1重组粘粒与DNA末端蛋白复合物混合后以磷酸钙共沉淀法转染2 93细胞。