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1·Methods:DNA sequencing and restriction endonuclease reaction was used.

方法：采用DNA测序及限制性酶切反应方法。

2·After a restriction endonuclease cleavage, two expected smaller fragments were observed.

经限制性内切酶反应，获得两个预期大小的片段。

3·The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。

4·Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.

然后对扩增产物进行限制性酶切反应；

5·In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。

6·Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the AGAR gel electrophoresis.

最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。

7·Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.

结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。

8·Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

结果经pcr方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。

9·Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;

并对发现突变的基因进行分析。方法 应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；

10·Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

方法用盐提取法提取人群中白细胞的DNA，以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。