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1·The column must be pre-equilibrated in binding buffer before beginning sample application.

列必须在开端抽样应用之前在捆绑的缓冲器中被平衡。

2·After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.

包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。

3·Gets or sets the maximum amount of memory allocated for the buffer manager that manages the buffers required by endpoints using this binding.

获取或设置可为缓冲区管理器分配的最大内存量，该管理器管理使用此绑定的终结点所需的缓冲区。

4·We furthermore found that an appropriate buffer ion can intensify the binding although its concentration and acidity remain constant (8).

我们进一步发现适合的缓冲离子能强化结合，虽然它的浓度和酸度都保持不变。