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1·The retention volume of targeted compounds was predicted in the step gradient elution conditions.

预测了台阶梯度洗脱条件下待分离物质的保留体积。

2·The results show that the new method leads to no excessive numerical wiggles and results in effective estimation of retention volume in each cell with shallow water.

结果显示，本文的干湿判断方法无明显的数值振荡产生，且可以较准确的估计浅水处水体的蓄留能力。

3·CONCLUSION: in a given column volume of gelatin, the quantity of purified fibronectin was closely related with the plasma retention time in column and the total loading amount of plasma.

结论在明胶亲和柱体积不变的条件下，制备纤维蛋白结合素的量与血浆加样量、血浆在柱内停留时间密切相关。