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1·Objective To improve the primary cell culture methods of colorectal adenomas.

目的对大肠腺瘤细胞原代培养的方法作进一步的改进。

2·However, the primary cell culture methods which are employed now have many defects.

然而，目前正被应用的滋养层细胞分离、培养、纯化方法均存在一定程度的缺陷。

3·Primary cell culture was made from 23 malignant trophoblastic tumors and 19 normal early pregnant villi.

本文对23例恶性滋养细胞和19例正常早孕绒毛滋养细胞进行了原代培养。

4·We further developed the primary cell culture if the tumor cells which would lead to better use of this genetic model.

同时还建立了原代培养的肿瘤细胞，提升了模型的利用效率。

5·We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.

为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。

6·Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.

方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。

7·Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.

目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。

8·Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.

方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。

9·Objective: To further investigate the mechanisms of hair cell generation or regeneration, the primary culture systems of cochlear sensory epithelial cell (CSEC) of rats were established.

目的：建立大鼠耳蜗感觉上皮细胞(CSEC)的原代培养体系，为研究毛细胞再生的分子机制提供大量来源一致的细胞。

10·Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.

目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。