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1·Screening and sequencing present 120 valid sequences, 84 of them belong in expressed sequence tags (ESTs).

通过克隆鉴定和序列测定，获得120个有效片段，其中84个认定为序列表达标签（EST）。

2·The technology of expressed sequence tags (ESTs) was broadly applied in analysis of gene function and expression pattern.

表达序列标签）测序分析技术广泛应用于基因功能和表达模式的分析研究。

3·These ESTs were classified into 11 different functional categories, the highest proportion of which was related to protein synthesis.

同源序列功能分类大致分为11类，与蛋白质合成有关的蛋白所占比例最大。

4·The majority of these ESTs were co-expressed in response to desiccation and osmotic stress and were generally not induced in response to heat and cold stress.

这些EST中的大多数在对干旱和渗透压胁迫发生反应时是共表达的，而在耐受冷热胁迫时并不被诱导表达。

5·Conclusion It is a feasible method to discover and acquire novel genes of S. japonicum by ESTs, combination of primer-walking sequncing and bioinformatics technique.

结论表达序列标签法、步移法测序和生物信息学技术有利于发现日本血吸虫新基因。

6·We picked out 53 ESTs to make sequence analysis and homology blast and found that 28 ESTs was 100% homologous and 25 ESTs was different but high homologous to reported genes.

并从中筛选出了53个EST片段对其进行了序列分析和同源性检索，其中28个EST片段和报道的基因有100%同源性，有25个EST片段和报道过的基因有差异但高度同源。

7·BLAST analysis revealed that 19 ESTs had high homology with known genes in GenBank involved in defense response, stress reaction, disease defense detoxification and signal transduction.

BLAST分析表明，其中19个EST序列与已知功能基因序列同源性很高，分别参与了植物的防卫反应、胁迫反应、细胞解毒和信号转导等。